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KMID : 0644120140270020012
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Journal of Oriental Gynecology
2014 Volume.27 No. 2 p.12 ~ p.22
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Effects of Cuscutae Semen Water Extract on Apoptosis of MCF-7 Human Breast Cancer Cells
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Kim Ji-Hyun
Jung Eun-Hye
Yoo Dong-Youl
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Abstract
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Objectives: This study aimed to evaluate the effects of Cuscutae Semen water extract (CS) on MCF-7 human breast cancer cells.
Methods: To clarify the results, we cultivated MCF-7 cells in cell culture plates. And then we extracted each of 100 μg/ml, 300 μg/ml, 600 μg/ml CS, gave it to MCF-7 cell. After these process we performed MTT assay to elucidate the ability of apoptosis. The result of mRNA was analyzed by RT-PCR.
Results: Each of concentrated extracts CS decreased the survival rate of MCF-7 cells. CS decreased Bcl-2 which is known as a blocking cell apoptosis. Bax, caspase-3, P21 and RIP-1 that accelerate apoptogenic activity factors increased by CS. CS did not change the condition of caspase-8, caspase-9, P53 factors on MCF-7 cells. Furthermore caspase-8, caspase-9, P53 factors on MCF-7 cells does not make it more active but turn it on.
Conclusions: According to the above results, we could suggest that CS can occur the apoptosis on MCF-7 cells.
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KEYWORD
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Cuscutae Semen, Breast Cancer, Apoptosis, MCF-7 Cell, Bcl-2, Bax, Caspase-3, P21, RIP-1
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